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Addgene

pOAD
(Plasmid #196825)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196825 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    YCplac111
  • Backbone size (bp) 8429
  • Vector type
    Yeast Expression
  • Promoter ADH
  • Selectable markers
    LEU2
  • Tag / Fusion Protein
    • Gal4 Activation domain (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

  • Supplemental Documents

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reference for additional info : High-throughput screening for protein-protein interactions using two-hybrid assay. Methods Enzymol (2000) 328:3-14
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How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOAD was a gift from Stan Fields (Addgene plasmid # 196825 ; http://n2t.net/addgene:196825 ; RRID:Addgene_196825)

  • For your References section:

    A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M, Johnston M, Fields S, Rothberg JM. Nature. 2000 Feb 10;403(6770):623-7. doi: 10.1038/35001009. 10.1038/35001009 PubMed 10688190
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