pGEL669
(Plasmid
#196817)
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Purpose(Empty Backbone) LrCas9 genome editing plasmid in tomato
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZHY988
- Backbone size (bp) 15245
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Vector typePlant Expression, CRISPR, Synthetic Biology
- Promoter SlEF1a
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Selectable markersBasta
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEL669 was a gift from Yong Zhang (Addgene plasmid # 196817 ; http://n2t.net/addgene:196817 ; RRID:Addgene_196817) -
For your References section:
Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system. Zhong Z, Liu G, Tang Z, Xiang S, Yang L, Huang L, He Y, Fan T, Liu S, Zheng X, Zhang T, Qi Y, Huang J, Zhang Y. Nat Commun. 2023 Sep 29;14(1):6102. doi: 10.1038/s41467-023-41802-9. 10.1038/s41467-023-41802-9 PubMed 37773156