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Addgene

pCMV-BFP-U6-sgRNA
(Plasmid #196725)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196725 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tracrRNA sequnece: GTTTaAGAGCTAtgctgGAAAcagcaTAGCAAGTTtAAATAAGGCTagtccgttatcaacttgaaaaagtggcaccgagtcggtgc.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-BFP-U6-sgRNA was a gift from Bernhard Schmierer (Addgene plasmid # 196725 ; http://n2t.net/addgene:196725 ; RRID:Addgene_196725)
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