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pRDA174_EF1a_EnAsCas12a_BFP
(Plasmid #196724)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196724 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRDA_174 (#136476)
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Blasticidin ; BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    enASCas12a-HA-T2A-BSD-TagBFP
  • Species
    Acidaminococcus sp.
  • Mutation
    E174R, S542R, K548R
  • Promoter EF1a
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • Nucleoplasmin NLS - HA (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRDA174_EF1a_EnAsCas12a_BFP was a gift from Bernhard Schmierer (Addgene plasmid # 196724 ; http://n2t.net/addgene:196724 ; RRID:Addgene_196724)
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