pRDA52_Lenti.puro.U6_modified DR
(Plasmid
#196723)
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Purpose(Empty Backbone) For cloning pairs of AsCas12a guides with two DRs that are as different as possible.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonelentiGuide-Puro (#52963)
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Vector typeLentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Modified pRDA_052 to change DR from TAATTTCTACTCTTGTAGAT to AAATTTCTCCTCTTGGAGAT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRDA52_Lenti.puro.U6_modified DR was a gift from Bernhard Schmierer (Addgene plasmid # 196723 ; http://n2t.net/addgene:196723 ; RRID:Addgene_196723)