lenti-Cas9-Exo-Blast-BFP
(Plasmid
#196721)
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PurposeSortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelenti dCas9-VP64_Blast (#61425)
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Backbone manufacturerFeng Zhang Lab
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Vector typeLentiviral, CRISPR
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Selectable markersBlasticidin ; BFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCas9-Exo1-T2A-BSD-TagBFP
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SpeciesS. pyogenes, E.coli
- Promoter EF1a
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Tags
/ Fusion Proteins
- Nucleoplasmin NLS (N terminal on insert)
- SV40 NLS (C terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lenti-Cas9-Exo-Blast-BFP was a gift from Bernhard Schmierer (Addgene plasmid # 196721 ; http://n2t.net/addgene:196721 ; RRID:Addgene_196721)
