CROPSeq-PP7-p65-HSF
(Plasmid
#196719)
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Purpose(Empty Backbone) CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196719 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCROPseq-Guide-Puro (#86708)
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Backbone manufacturerChristoph Bock Lab
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Vector typeLentiviral, CRISPR
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Selectable markersPuromycin
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Tags
/ Fusion Proteins
- Nucleoplasmin NLS (N terminal on backbone)
- SV40 NLS (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- Nucleoplasmin NLS (N terminal on backbone)
- SV40 NLS (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Guide cloning with BsmBI and T4 Ligase, do not use Gibson assembly as the plasmid is prone to recombination
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROPSeq-PP7-p65-HSF was a gift from Bernhard Schmierer (Addgene plasmid # 196719 ; http://n2t.net/addgene:196719 ; RRID:Addgene_196719)
