Skip to main content
Addgene

CROPSeq-BFP
(Plasmid #196718)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196718 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    CROPseq-Guide-Puro (#86708)
  • Backbone manufacturer
    Christoph Bock Lab
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin ; BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tracrRNA sequnece: GTTTaAGAGCTAtgctgGAAAcagcaTAGCAAGTTtAAATAAGGCTagtccgttatcaacttgaaaaagtggcaccgagtcggtgc. Guide cloning with BsmBI and T4 Ligase, do not use Gibson assembly as the plasmid is prone to recombination

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CROPSeq-BFP was a gift from Bernhard Schmierer (Addgene plasmid # 196718 ; http://n2t.net/addgene:196718 ; RRID:Addgene_196718)
Related items:
Commonly requested with: