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lenti-UCOE-dCas9-BFP-VP64
(Plasmid #196717)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196717 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti dCas9-VP64_Blast (#61425)
  • Backbone manufacturer
    Feng Zhang Lab
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-TagBFP-VP64
  • Species
    S. pyogenes
  • Mutation
    D10A, N863A
  • Promoter EF1a
  • Tags / Fusion Proteins
    • Nucleoplasmin NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

contains a ubiquitous chromatin opening element

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-UCOE-dCas9-BFP-VP64 was a gift from Bernhard Schmierer (Addgene plasmid # 196717 ; http://n2t.net/addgene:196717 ; RRID:Addgene_196717)
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