lenti.Cas9.BFP.sgRNA.parental
(Plasmid #196715)

• Purpose: (Empty Backbone) Sortable WT-SpCas9 expression and guide cassette (1-vector system).
• Depositing Lab: Bernhard Schmierer
• Publication: Schmierer unpublished plasmids (unpublished)

Backbone

• Vector backbone: lenti dCas9-VP64_Blast (#61425)
• Backbone manufacturer: Feng Zhang Lab
• Vector type: Lentiviral, CRISPR
• Selectable markers: BFP
• Tags / Fusion Proteins:
  • Nucleoplasmin NLS (N terminal on backbone)
  • SV40 NLS (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • Nucleoplasmin NLS (N terminal on backbone)
  • SV40 NLS (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry