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lenti-Cas9-sgHPRT1
(Plasmid #196713)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lenti dCas9-VP64_Blast (#61425)
  • Backbone manufacturer
    Feng Zhang Lab
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas9-T2A-BSD-U6-sgHPRT1
  • gRNA/shRNA sequence
    ATGTGATGAAGGAGATGGG
  • Species
    S. pyogenes
  • Promoter EF1a/hU6
  • Tags / Fusion Proteins
    • Nucleoplasmin NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti-Cas9-sgHPRT1 was a gift from Bernhard Schmierer (Addgene plasmid # 196713 ; http://n2t.net/addgene:196713 ; RRID:Addgene_196713)

  • For your References section:

    CRISPR/Cas9 screening using unique molecular identifiers. Schmierer B, Botla SK, Zhang J, Turunen M, Kivioja T, Taipale J. Mol Syst Biol. 2017 Oct 9;13(10):945. doi: 10.15252/msb.20177834. 10.15252/msb.20177834 PubMed 28993443
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