lenti-dCas9-ZIM3-KRAB-BFP
(Plasmid #196712)

• Purpose: CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
• Depositing Lab: Bernhard Schmierer
• Publication: Schmierer unpublished plasmids (unpublished)

Backbone

• Vector backbone: lenti dCas9-VP64_Blast (#61425)
• Backbone manufacturer: Feng Zhang Lab
• Vector type: Lentiviral, CRISPR
• Selectable markers: BFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dCas9-KRAB(ZIM3)-T2A-TagBFP
• Species: S. pyogenes
• Mutation: D10A, N863A
• Promoter: EF1a
• Tags / Fusion Proteins:
  • Nucleoplasmin NLS (N terminal on insert)
  • SV40 NLS (C terminal on insert)

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • lenti-dCas9-ZIM3-KRAB-BFP.gb
• A portion of this plasmid was derived from a plasmid made by: Contains KRAB domain from ZIM3: Alerasool N, et al. An efficient KRAB domain for CRISPRi applications in human cells. Nat Methods. 2020 doi: 10.1038/s41592-020-0966-x. PMID: 33020655.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry