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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI
(Plasmid #196710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiGuide-Puro (#52963)
  • Backbone manufacturer
    Feng Zhang Lab
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

tracrRNA sequence: GTTTaAGAGCTAtgctgGAAAcagcaTAGCAAGTTtAAATAAGGCTagtccgttatcaacttgaaaaagtggcaccgagtcggtgc.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiGuide-Puro-Chen-tracrRNA_2xBsmBI was a gift from Bernhard Schmierer (Addgene plasmid # 196710 ; http://n2t.net/addgene:196710 ; RRID:Addgene_196710)

  • For your References section:

    CRISPR/Cas9 screening using unique molecular identifiers. Schmierer B, Botla SK, Zhang J, Turunen M, Kivioja T, Taipale J. Mol Syst Biol. 2017 Oct 9;13(10):945. doi: 10.15252/msb.20177834. 10.15252/msb.20177834 PubMed 28993443
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