lentiGuide-Puro.3xBsmBI
(Plasmid
#196709)
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Purpose(Empty Backbone) Backbone for cloning guide libraries with untranscribed barcodes and a tracrRNA of choice
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196709 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelentiGuide-Puro (#52963)
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Backbone manufacturerFeng Zhang Lab
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Vector typeLentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
lentiGuide-Puro.3xBsmBI was a gift from Bernhard Schmierer (Addgene plasmid # 196709 ; http://n2t.net/addgene:196709 ; RRID:Addgene_196709) -
For your References section:
CRISPR/Cas9 screening using unique molecular identifiers. Schmierer B, Botla SK, Zhang J, Turunen M, Kivioja T, Taipale J. Mol Syst Biol. 2017 Oct 9;13(10):945. doi: 10.15252/msb.20177834. 10.15252/msb.20177834 PubMed 28993443
