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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)
(Plasmid #196703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human RNaseH1 D210N
  • Species
    E. coli
  • Mutation
    D210N, catalytically inactive

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N) was a gift from Karlene Cimprich (Addgene plasmid # 196703 ; http://n2t.net/addgene:196703 ; RRID:Addgene_196703)

  • For your References section:

    R-loop-derived cytoplasmic RNA-DNA hybrids activate an immune response. Crossley MP, Song C, Bocek MJ, Choi JH, Kousorous J, Sathirachinda A, Lin C, Brickner JR, Bai G, Lans H, Vermeulen W, Abu-Remaileh M, Cimprich KA. Nature. 2023 Jan;613(7942):187-194. doi: 10.1038/s41586-022-05545-9. Epub 2022 Dec 21. 10.1038/s41586-022-05545-9 PubMed 36544021
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