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Addgene

kiCAP-AAV-M.Fas.3
(Plasmid #196689)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196689 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-Kan
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 9398
  • Modifications to backbone
    Backbone modifications as in kiCAP-AAV-BI30
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
  • Alt name
    M.Fas.3
  • Species
    Synthetic
  • Insert Size (bp)
    2232
  • Mutation
    RVDPSGL insert between amino acids 588 and 589 of AAV9 VP1
  • Promoter p41

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsiWI (not destroyed)
  • 3′ cloning site AgeI (destroyed during cloning)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    kiCAP-AAV-M.Fas.3 was a gift from Pardis Sabeti (Addgene plasmid # 196689 ; http://n2t.net/addgene:196689 ; RRID:Addgene_196689)

  • For your References section:

    Systemic administration of novel engineered AAV capsids facilitates enhanced transgene expression in the macaque CNS. Stanton AC, Lagerborg KA, Tellez L, Krunnfusz A, King EM, Ye S, Solomon IH, Tabebordbar M, Sabeti PC. Med (N Y). 2022 Nov 15:S2666-6340(22)00456-1. doi: 10.1016/j.medj.2022.11.002. 10.1016/j.medj.2022.11.002 PubMed 36417917
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