pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE
(Plasmid
#196678)
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PurposeAAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196678 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV w CAG promotor, FLEX cassette and WPRE
- Total vector size (bp) 6067
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePKIαmut-P2A-mRuby3
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SpeciesSynthetic
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Insert Size (bp)2334
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE was a gift from Haining Zhong (Addgene plasmid # 196678 ; http://n2t.net/addgene:196678 ; RRID:Addgene_196678) -
For your References section:
Locomotion activates PKA through dopamine and adenosine in striatal neurons. Ma L, Day-Cooney J, Benavides OJ, Muniak MA, Qin M, Ding JB, Mao T, Zhong H. Nature. 2022 Nov;611(7937):762-768. doi: 10.1038/s41586-022-05407-4. Epub 2022 Nov 9. 10.1038/s41586-022-05407-4 PubMed 36352228