pAAV_SynP_DIO_tAKARα- T391A_WPRE
(Plasmid
#196676)
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PurposeAAV plasmid for mammalian Cre-dependent expression of the T391A mutant of tAKARα (abolished sensor phosphorylation site).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196676 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV w SynP, DIO cassette and WPRE
- Total vector size (bp) 7417
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametAKARα-T391A
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SpeciesSynthetic
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Insert Size (bp)2562
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_SynP_DIO_tAKARα- T391A_WPRE was a gift from Haining Zhong (Addgene plasmid # 196676 ; http://n2t.net/addgene:196676 ; RRID:Addgene_196676) -
For your References section:
Locomotion activates PKA through dopamine and adenosine in striatal neurons. Ma L, Day-Cooney J, Benavides OJ, Muniak MA, Qin M, Ding JB, Mao T, Zhong H. Nature. 2022 Nov;611(7937):762-768. doi: 10.1038/s41586-022-05407-4. Epub 2022 Nov 9. 10.1038/s41586-022-05407-4 PubMed 36352228