pIV101
(Plasmid #196671)

• Purpose: (Empty Backbone) Enables recombination mediated gene delivery into Agrobacterium strains for subsequent transformation of plants
• Depositing Lab: Simona Radutoiu
• Publication: Ferguson et al PLoS One. 2023 Nov 1;18(11):e0291680. doi: 10.1371/journal.pone.0291680. eCollection 2023.

Backbone

• Vector backbone: pIV101
• Backbone size (bp): 4790
• Vector type: Integration vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Spectinomycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pIV101 for addgene.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pIV101 is a level 2 destination vector (pL2-1) based on the MoClo Golden Gate Assembly (GGA) strategy published by Weber et al., 2011 (https://doi.org/10.1371/journal.pone.0016765) For level 2 cloning the GGA cloning site contains TGCC and GGGA fusion sites generated by digestion with BpiI (BbsI). The GGA cloning site also contains nested BsaI restriction sites which result in GGAG and CGCT fusion sites, compatible with insertion of level 1 fragments. The GGA site contains lacZa enabling blue/white selection in E. coli strains carrying the lacZΔM15 mutation.