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Addgene

pWzyE-strep-mClover3
(Plasmid #196668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD24
  • Backbone size w/o insert (bp) 4617
  • Total vector size (bp) 6846
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Protein expression in E. coli C43(DE3).
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WzyE
  • Species
    Pectobacterium atrosepticum
  • Insert Size (bp)
    2229
  • GenBank ID
    57210867
  • Promoter araBAD
  • Tags / Fusion Proteins
    • TEV-cleavable di-strep tag II (C terminal on insert)
    • mClover3 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBAD forward
  • 3′ sequencing primer pBAD reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWzyE-strep-mClover3 was a gift from James Naismith (Addgene plasmid # 196668 ; http://n2t.net/addgene:196668 ; RRID:Addgene_196668)

  • For your References section:

    The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex. Weckener M, Woodward LS, Clarke BR, Liu H, Ward PN, Le Bas A, Bhella D, Whitfield C, Naismith JH. Open Biol. 2023 Mar;13(3):220373. doi: 10.1098/rsob.220373. Epub 2023 Mar 22. 10.1098/rsob.220373 PubMed 36944376
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