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pB_tetO7_sfGFP
(Plasmid #196616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196616 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HO-poly-KanMX4-HO (ATCC 87804)
  • Backbone manufacturer
    ATCC
  • Backbone size w/o insert (bp) 4573
  • Total vector size (bp) 9527
  • Vector type
    Yeast Expression
  • Selectable markers
    KanMX (select with Geneticin/G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    NEB5a strain
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Superfolder GFP
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    4954
  • Promoter tetO7 promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AATTATCCTGGGCACGAG
  • 3′ sequencing primer ACTGTAAGATTCCGCCAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Supplementary Table 2 of the paper provides details on the cloning of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB_tetO7_sfGFP was a gift from Matthias Heinemann (Addgene plasmid # 196616 ; http://n2t.net/addgene:196616 ; RRID:Addgene_196616)

  • For your References section:

    Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle. Takhaveev V, Ozsezen S, Smith EN, Zylstra A, Chaillet ML, Chen H, Papagiannakis A, Milias-Argeitis A, Heinemann M. Nat Metab. 2023 Feb;5(2):294-313. doi: 10.1038/s42255-023-00741-x. Epub 2023 Feb 27. 10.1038/s42255-023-00741-x PubMed 36849832
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