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pROS_phleo-Tps1/Gsy2
(Plasmid #196612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pROS13
  • Modifications to backbone
    The kanMX cassette in pROS13 was replaced by the pAgTEF1-ble-tAgTEF1 cassette from pUG66 conveying phleomycin resistance.
  • Vector type
    Yeast Expression
  • Selectable markers
    Phleomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TPS1, GSY2
  • gRNA/shRNA sequence
    TPS1: TACAATAATAGCACCATTCA, GSY2: AATTTGTAAAAAAGACAAGA
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    TPS1 (a.k.a. YBR126C, BYP1, CIF1, FDP1, GGS1, GLC6, TSS1)
  • Entrez Gene
    GSY2 (a.k.a. YLR258W)
  • Promoter SNR52

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CACCTTTCGAGAGGACGATG
  • 3′ sequencing primer GCTGGCCTTTTGCTCACATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloning details are in the Methods section and Supplementary Table 2 of the paper.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pROS_phleo-Tps1/Gsy2 was a gift from Matthias Heinemann (Addgene plasmid # 196612 ; http://n2t.net/addgene:196612 ; RRID:Addgene_196612)

  • For your References section:

    Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle. Takhaveev V, Ozsezen S, Smith EN, Zylstra A, Chaillet ML, Chen H, Papagiannakis A, Milias-Argeitis A, Heinemann M. Nat Metab. 2023 Feb;5(2):294-313. doi: 10.1038/s42255-023-00741-x. Epub 2023 Feb 27. 10.1038/s42255-023-00741-x PubMed 36849832
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