Skip to main content
Addgene

FLAG-MST1(K59R)
(Plasmid #1966)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1966 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV5
  • Backbone size w/o insert (bp) 4657
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MST1
  • Alt name
    stk4
  • Species
    M. musculus (mouse)
  • Mutation
    K59R (kinase inactive); N295D
  • Entrez Gene
    Stk4 (a.k.a. AI447339, AU020804, Kas, Kas-2, Mst, Mst1, Ysk, Ysk3)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    cDNA from Leonard Zon (Children's Hospital, Boston)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Kinase inactive due to mutation at the ATP binding site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-MST1(K59R) was a gift from Joseph Avruch (Addgene plasmid # 1966)

  • For your References section:

    Death-associated protein 4 binds MST1 and augments MST1-induced apoptosis. Lin Y, Khokhlatchev A, Figeys D, Avruch J. J Biol Chem 2002 Dec 13;277(50):47991-8001. Epub 2002 Oct 15. 10.1074/jbc.M202630200 PubMed 12384512
Related items: