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Addgene

Td-CGBE
(Plasmid #196599)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196599 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
  • Insert Size (bp)
    4818
  • Mutation
    TadA-8e (N46L); S. pyogenes Cas9 (D10A)
  • Tag / Fusion Protein
    • 6*His (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Td-CGBE was a gift from Dali Li (Addgene plasmid # 196599 ; http://n2t.net/addgene:196599 ; RRID:Addgene_196599)

  • For your References section:

    Re-engineering the adenine deaminase TadA-8e for efficient and specific CRISPR-based cytosine base editing. Chen L, Zhu B, Ru G, Meng H, Yan Y, Hong M, Zhang D, Luan C, Zhang S, Wu H, Gao H, Bai S, Li C, Ding R, Xue N, Lei Z, Chen Y, Guan Y, Siwko S, Cheng Y, Song G, Wang L, Yi C, Liu M, Li D. Nat Biotechnol. 2022 Nov 10. doi: 10.1038/s41587-022-01532-7. 10.1038/s41587-022-01532-7 PubMed 36357717
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