pDONR201-Eps8L2_181-299
(Plasmid
#196528)
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PurposeEntry vector for Gateway with Eps8L2_181-299
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR201
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEps8L2_181-299
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SpeciesH. sapiens (human)
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Insert Size (bp)357
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Entrez GeneEPS8L2 (a.k.a. EPS8R2, MGC3088, FLJ16738, FLJ21935, FLJ22171, MGC126530)
- Promoter CMV promoter; T7 promoter
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR201-Eps8L2_181-299 was a gift from Edgar Gomes (Addgene plasmid # 196528 ; http://n2t.net/addgene:196528 ; RRID:Addgene_196528) -
For your References section:
Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration. Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER. Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 10.1016/j.cub.2021.01.007. Epub 2021 Feb 9. 10.1016/j.cub.2021.01.007 PubMed 33567288