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pcDNA3.1+CeGFP-Ctdnep1WTsiR
(Plasmid #196517)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196517 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1+EeGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ctdnep1WTsiR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    797
  • Mutation
    Silent mutations for siRNA resistance
  • Entrez Gene
    CTDNEP1 (a.k.a. DULLARD, HSA011916, NET56)
  • Promoter CMV promoter; T7 promoter
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains attb1 and attb2 sites and a Linker sequence between Ctdnep1 and eGFP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1+CeGFP-Ctdnep1WTsiR was a gift from Edgar Gomes (Addgene plasmid # 196517 ; http://n2t.net/addgene:196517 ; RRID:Addgene_196517)

  • For your References section:

    Ctdnep1 and Eps8L2 regulate dorsal actin cables for nuclear positioning during cell migration. Calero-Cuenca FJ, Osorio DS, Carvalho-Marques S, Sridhara SC, Oliveira LM, Jiao Y, Diaz J, Janota CS, Cadot B, Gomes ER. Curr Biol. 2021 Apr 12;31(7):1521-1530.e8. doi: 10.1016/j.cub.2021.01.007. Epub 2021 Feb 9. 10.1016/j.cub.2021.01.007 PubMed 33567288
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