-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 1965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV5
- Backbone size w/o insert (bp) 4657
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameMST1
-
Alt namestk4
-
SpeciesM. musculus (mouse)
-
Entrez GeneStk4 (a.k.a. AI447339, AU020804, Kas, Kas-2, Mst, Mst1, Ysk, Ysk3)
-
Tag
/ Fusion Protein
- FLAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bycDNA from Leonard Zon (Children's Hospital, Boston)
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FLAG-MST1 was a gift from Joseph Avruch (Addgene plasmid # 1965 ; http://n2t.net/addgene:1965 ; RRID:Addgene_1965) -
For your References section:
Death-associated protein 4 binds MST1 and augments MST1-induced apoptosis. Lin Y, Khokhlatchev A, Figeys D, Avruch J. J Biol Chem 2002 Dec 13;277(50):47991-8001. Epub 2002 Oct 15. 10.1074/jbc.M202630200 PubMed 12384512