pcPylRS(Y384F Y306A)
(Plasmid
#196486)
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PurposeTo express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1/Zeo(+)
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Backbone manufacturerThermo Fisher (Invitrogen)
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
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SpeciesMethanosarcina mazei
- Promoter CMV
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcPylRS(Y384F Y306A) was a gift from Kensaku Sakamoto (Addgene plasmid # 196486 ; http://n2t.net/addgene:196486 ; RRID:Addgene_196486) -
For your References section:
Site-specific incorporation of unnatural amino acids into proteins in mammalian cells. Hino N, Sakamoto K, Yokoyama S. Methods Mol Biol. 2012;794:215-28. doi: 10.1007/978-1-61779-331-8_13. 10.1007/978-1-61779-331-8_13 PubMed 21956565
