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pcpBpaRS_v2
(Plasmid #196485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
  • Species
    E. coli (synthetase), Bacillus stearothermophilus (tRNA)
  • Promoter SV40(synthetase), Pol III tRNA promoter (tRNA), CMV for additional expression of recombinant proteins

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcpBpaRS_v2 was a gift from Kensaku Sakamoto (Addgene plasmid # 196485 ; http://n2t.net/addgene:196485 ; RRID:Addgene_196485)

  • For your References section:

    Site-specific incorporation of unnatural amino acids into proteins in mammalian cells. Hino N, Sakamoto K, Yokoyama S. Methods Mol Biol. 2012;794:215-28. doi: 10.1007/978-1-61779-331-8_13. 10.1007/978-1-61779-331-8_13 PubMed 21956565
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