pcpAzpaRS_v2
(Plasmid #196483)

• Purpose: To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
• Depositing Lab: Kensaku Sakamoto
• Publication: Hino et al Methods Mol Biol. 2012;794:215-28. doi: 10.1007/978-1-61779-331-8_13.

Backbone

• Vector backbone: pcDNA4/TO
• Backbone manufacturer: Thermo Fisher (Invitrogen)
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
• Species: E. coli (synthetase), Bacillus stearothermophilus (tRNA)
• Promoter: SV40(synthetase), Pol III tRNA promoter (tRNA), CMV for additional expression of recombinant proteins

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: Unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthetic

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: This plasmid contains G37L, G182S and F183M mutations in amber suppressor tRNA. These mutations are not known to affect plasmid function.