pQE80l B-CN-B
(Plasmid
#196477)
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PurposeProtein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCN
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQE80l
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameB-CN-B
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Alt nameSpyCatcher-ELP-CarHCN-ELP-SpyCatcher
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SpeciesOther
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE80l B-CN-B was a gift from Fei Sun (Addgene plasmid # 196477 ; http://n2t.net/addgene:196477 ; RRID:Addgene_196477) -
For your References section:
B(12)-induced reassembly of split photoreceptor protein enables photoresponsive hydrogels with tunable mechanics. Yang Z, Fok HKF, Luo J, Yang Y, Wang R, Huang X, Sun F. Sci Adv. 2022 Apr;8(13):eabm5482. doi: 10.1126/sciadv.abm5482. Epub 2022 Apr 1. 10.1126/sciadv.abm5482 PubMed 35363531