p32m3C Trx-CC
(Plasmid
#196475)
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PurposeProtein expression of Thiolredoxin-3C cleavage site-CarHCC
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep32m3C
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrx-CC
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Alt nameThioredoxin tag-3C cleavage site-CarHCN
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SpeciesOther
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p32m3C Trx-CC was a gift from Fei Sun (Addgene plasmid # 196475 ; http://n2t.net/addgene:196475 ; RRID:Addgene_196475) -
For your References section:
B(12)-induced reassembly of split photoreceptor protein enables photoresponsive hydrogels with tunable mechanics. Yang Z, Fok HKF, Luo J, Yang Y, Wang R, Huang X, Sun F. Sci Adv. 2022 Apr;8(13):eabm5482. doi: 10.1126/sciadv.abm5482. Epub 2022 Apr 1. 10.1126/sciadv.abm5482 PubMed 35363531