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pETDuet-MerTK_KRmutant-PTPN1
(Plasmid #196447)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196447 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETduet1
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    Thrombin cleavage site inserted
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Tyrosine-Protein Kinase Mer
  • Alt name
    MERTK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    885
  • Mutation
    K591R, K639R, K702R, K865R
  • Entrez Gene
    MERTK (a.k.a. MER, RP38, Tyro12, c-Eyk, c-mer)
  • Promoter T7 promoter

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pETupstream
  • 3′ sequencing primer DuetDOWN1
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    PTPN1
  • Alt name
    PTP1B
  • Species
    H. sapiens (human)
  • Entrez Gene
    PTPN1 (a.k.a. PTP1B)
  • Promoter T7 promoter

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site Acc65I (not destroyed)
  • 5′ sequencing primer DuetUP2
  • 3′ sequencing primer T7terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETDuet-MerTK_KRmutant-PTPN1 was a gift from Byung Il Lee (Addgene plasmid # 196447 ; http://n2t.net/addgene:196447 ; RRID:Addgene_196447)

  • For your References section:

    BMS794833 inhibits macrophage efferocytosis by directly binding to MERTK and inhibiting its activity. Bae SH, Kim JH, Park TH, Lee K, Lee BI, Jang H. Exp Mol Med. 2022 Sep;54(9):1450-1460. doi: 10.1038/s12276-022-00840-x. Epub 2022 Sep 2. 10.1038/s12276-022-00840-x PubMed 36056187
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