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AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE
(Plasmid #196414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3406
  • Total vector size (bp) 6644
  • Modifications to backbone
    The GfaABC1D promoter and the 4x6T miRNA targeting cassette were added to confer astrocyte specificity.
  • Vector type
    Mammalian Expression, AAV ; Astrocyte-selective

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Lck-smFLAG
  • Alt name
    membrane targeted FLAG spaghetti monster
  • Alt name
    smFP_FLAG
  • Species
    Synthetic
  • Insert Size (bp)
    1158
  • Promoter GfaABC1D
  • Tag / Fusion Protein
    • Lck (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tagcccactccttcataaag
  • 3′ sequencing primer ttattaggacaaggctggtg
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    AAV-GfaABC1D-lck-smFLAG-4x6T was created using AAV pmSyn1-EBFP-Cre (Addgene 51507) provided by Hongkui Zeng and smFLAG (Addgene 59756) provided by Loren Looger.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-GfaABC1D-lck-smFLAG-4x6T-WPRE was a gift from Stanley Thomas Carmichael (Addgene plasmid # 196414 ; http://n2t.net/addgene:196414 ; RRID:Addgene_196414)

  • For your References section:

    A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences. Gleichman AJ, Kawaguchi R, Sofroniew MV, Carmichael ST. Nat Commun. 2023 Nov 16;14(1):7426. doi: 10.1038/s41467-023-42746-w. 10.1038/s41467-023-42746-w PubMed 37973910
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