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Addgene

NK809
(Plasmid #196406)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196406 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
  • Species
    Synthetic
  • Mutation
    N/A (synthetic)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.11.11.515474 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NK809 was a gift from Nicole King (Addgene plasmid # 196406 ; http://n2t.net/addgene:196406 ; RRID:Addgene_196406)

  • For your References section:

    An RFX transcription factor regulates ciliogenesis in the closest living relatives of animals. Coyle MC, Tajima AM, Leon F, Choksi SP, Yang A, Espinoza S, Hughes TR, Reiter JF, Booth DS, King N. Curr Biol. 2023 Aug 2:S0960-9822(23)00929-6. doi: 10.1016/j.cub.2023.07.022. 10.1016/j.cub.2023.07.022 PubMed 37552984
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