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pET28b_TypeIC_acrIC4_invivo
(Plasmid #196403)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196403 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Total vector size (bp) 5355
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-CRISPR IC4 protein
  • Species
    P. aeruginosa
  • Insert Size (bp)
    174
  • GenBank ID
    WP_1535753561.1
  • Promoter T7

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b_TypeIC_acrIC4_invivo was a gift from David Taylor (Addgene plasmid # 196403 ; http://n2t.net/addgene:196403 ; RRID:Addgene_196403)

  • For your References section:

    Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub 2023 Feb 16. 10.1016/j.molcel.2023.01.024 PubMed 36805026
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