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Addgene

pET28b_TypeIC_acrIF2_invivo
(Plasmid #196402)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Total vector size (bp) 5457
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    anti-CRISPR IF2 protein
  • Alt name
    AcrIF2
  • Species
    P. aeruginosa
  • Insert Size (bp)
    273
  • GenBank ID
    UniProt: Q6TM72 WP_015972868.1
  • Entrez Gene
    orf30 (a.k.a. D3112p30)
  • Promoter T7

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    gene cloned from addgene plasmid #89234

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b_TypeIC_acrIF2_invivo was a gift from David Taylor (Addgene plasmid # 196402 ; http://n2t.net/addgene:196402 ; RRID:Addgene_196402)

  • For your References section:

    Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub 2023 Feb 16. 10.1016/j.molcel.2023.01.024 PubMed 36805026
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