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pBAD_TypeIC_Cascade_cas3_invivo
(Plasmid #196400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD
  • Total vector size (bp) 9538
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas5c
  • Species
    Desulfovibrio vulgaris str. Hildenborough
  • Insert Size (bp)
    680
  • GenBank ID
    2781521 YP_009170
  • Promoter araBAD

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Cas7c
  • Species
    Desulfovibrio vulgaris str. Hildenborough
  • Insert Size (bp)
    873
  • GenBank ID
    2781595 YP_009172.1
  • Promoter araBAD

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Cas8c
  • Species
    Desulfovibrio vulgaris str. Hildenborough
  • Insert Size (bp)
    1839
  • GenBank ID
    2781582 YP_009171.1
  • Promoter araBAD

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    Cas3c
  • Species
    Desulfovibrio vulgaris str. Hildenborough
  • Insert Size (bp)
    2106
  • GenBank ID
    GenBank: AAS94335.1
  • Promoter araBAD
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information for Gene/Insert 4

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Cas8c/Cas5c/Cas7c were cloned from addgene plasmid #81185

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD_TypeIC_Cascade_cas3_invivo was a gift from David Taylor (Addgene plasmid # 196400 ; http://n2t.net/addgene:196400 ; RRID:Addgene_196400)

  • For your References section:

    Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. O'Brien RE, Bravo JPK, Ramos D, Hibshman GN, Wright JT, Taylor DW. Mol Cell. 2023 Mar 2;83(5):746-758.e5. doi: 10.1016/j.molcel.2023.01.024. Epub 2023 Feb 16. 10.1016/j.molcel.2023.01.024 PubMed 36805026
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