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pαH-S-GSAS-2P-N317P
(Plasmid #196378)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196378 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pαH
  • Backbone size w/o insert (bp) 4746
  • Total vector size (bp) 8367
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S-GSAS-2P-N317P
  • Species
    Severe acute respiratory syndrome coronavirus 2
  • Insert Size (bp)
    3624
  • Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RRAR) replaced with GSAS; N317P
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tags / Fusion Proteins
    • HRV 3C cleavage site (before tags) C terminal on backbone 8X His tag C and 2X Strep-Tag II C terminal on backbone (C terminal on backbone)
    • HRV3C cleavage site, 8X His tag, 2X Strep-Tag II (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A portion of this plasmid was derived from a plasmid made by Jason McLellan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pαH-S-GSAS-2P-N317P was a gift from Priyamvada Acharya (Addgene plasmid # 196378 ; http://n2t.net/addgene:196378 ; RRID:Addgene_196378)

  • For your References section:

    Network analysis uncovers the communication structure of SARS-CoV-2 spike protein identifying sites for immunogen design. Manrique PD, Chakraborty S, Henderson R, Edwards RJ, Mansbach R, Nguyen K, Stalls V, Saunders C, Mansouri K, Acharya P, Korber B, Gnanakaran S. iScience. 2023 Jan 20;26(1):105855. doi: 10.1016/j.isci.2022.105855. Epub 2022 Dec 26. 10.1016/j.isci.2022.105855 PubMed 36590900
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