RCAS.Sfi
(Plasmid
#196377)
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Purpose(Empty Backbone) Empty avian expression vector RCAS with modifications for fixed orientation cloning into SfiI sites
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196377 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneRCAS(BP-A)
- Backbone size (bp) 11600
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Modifications to backboneSfiI sites added for directional cloning
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Vector typeRetroviral ; avian
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RCAS.Sfi was a gift from Peter Vogt (Addgene plasmid # 196377 ; http://n2t.net/addgene:196377 ; RRID:Addgene_196377) -
For your References section:
The akt kinase: molecular determinants of oncogenicity. Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5. 10.1073/pnas.95.25.14950 PubMed 9843996