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Addgene

RCAS.Sfi Akt1-K179M
(Plasmid #196375)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196375 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCAS(BP-A)
  • Backbone size w/o insert (bp) 11600
  • Modifications to backbone
    SfiI sites added for directional cloning
  • Vector type
    Retroviral ; avian

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    c-akt1 K179M
  • Alt name
    kinase dead Akt1
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CP Tsichlis's Lab/Fox Chase Cancer Center

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS.Sfi Akt1-K179M was a gift from Peter Vogt (Addgene plasmid # 196375 ; http://n2t.net/addgene:196375 ; RRID:Addgene_196375)

  • For your References section:

    The akt kinase: molecular determinants of oncogenicity. Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5. 10.1073/pnas.95.25.14950 PubMed 9843996
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