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Addgene

RCAS myr-c-P3k
(Plasmid #196373)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196373 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCAS(BP-A)
  • Backbone size w/o insert (bp) 11600
  • Modifications to backbone
    SfiI sites added for directional cloning
  • Vector type
    Retroviral ; avian

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Myr-c-P3k-FLAG
  • Species
    G. gallus (chicken)
  • Tags / Fusion Proteins
    • myristoylation (N terminal on insert)
    • flag (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAS myr-c-P3k was a gift from Peter Vogt (Addgene plasmid # 196373 ; http://n2t.net/addgene:196373 ; RRID:Addgene_196373)

  • For your References section:

    Transformation of chicken cells by the gene encoding the catalytic subunit of PI 3-kinase. Chang HW, Aoki M, Fruman D, Auger KR, Bellacosa A, Tsichlis PN, Cantley LC, Roberts TM, Vogt PK. Science. 1997 Jun 20;276(5320):1848-50. doi: 10.1126/science.276.5320.1848. 10.1126/science.276.5320.1848 PubMed 9188528
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