pNIC28-SENP1
(Plasmid
#196354)
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Purposeprotein expression of the His-tagged catalytic domain
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepNIC28-Bsa4
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSENP1 catalytic domain
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SpeciesH. sapiens (human)
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Mutationcontains only E419-L644
- Promoter T7
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Tag
/ Fusion Protein
- His6-TEV (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC28-SENP1 was a gift from Stefan Knapp (Addgene plasmid # 196354 ; http://n2t.net/addgene:196354 ; RRID:Addgene_196354)