mEGFP-4xbiRhoBAST
(Plasmid
#196350)
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PurposeExpression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196350 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemAzurite-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemEGFP-4xbiRhoBAST
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned using mAzurite-C1 (#54583) . Please visit https://www.biorxiv.org/content/10.1101/2021.11.02.466936v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mEGFP-4xbiRhoBAST was a gift from Murat Sunbul (Addgene plasmid # 196350 ; http://n2t.net/addgene:196350 ; RRID:Addgene_196350) -
For your References section:
Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging. Buhler B, Schokolowski J, Benderoth A, Englert D, Grun F, Jaschke A, Sunbul M. Nat Chem Biol. 2023 Apr;19(4):478-487. doi: 10.1038/s41589-022-01228-8. Epub 2023 Jan 19. 10.1038/s41589-022-01228-8 PubMed 36658339