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Addgene

mEGFP-2xbiRhoBAST
(Plasmid #196349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mAzurite-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEGFP-2xbiRhoBAST
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned using mAzurite-C1 (#54583) . Please visit https://www.biorxiv.org/content/10.1101/2021.11.02.466936v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mEGFP-2xbiRhoBAST was a gift from Murat Sunbul (Addgene plasmid # 196349 ; http://n2t.net/addgene:196349 ; RRID:Addgene_196349)

  • For your References section:

    Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging. Buhler B, Schokolowski J, Benderoth A, Englert D, Grun F, Jaschke A, Sunbul M. Nat Chem Biol. 2023 Apr;19(4):478-487. doi: 10.1038/s41589-022-01228-8. Epub 2023 Jan 19. 10.1038/s41589-022-01228-8 PubMed 36658339
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