mAzurite-16xRhoBAST
(Plasmid
#196347)
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PurposeExpression of mAzurite mRNA with 16xRhoBAST in 3'UTR
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonemAzurite-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemAzurite-16xRhoBAST
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned using mAzurite-C1 (#54583). Please visit https://www.biorxiv.org/content/10.1101/2022.10.24.513449v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mAzurite-16xRhoBAST was a gift from Murat Sunbul (Addgene plasmid # 196347 ; http://n2t.net/addgene:196347 ; RRID:Addgene_196347) -
For your References section:
Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging. Englert D, Burger EM, Grun F, Verma MS, Lackner J, Lampe M, Buhler B, Schokolowski J, Nienhaus GU, Jaschke A, Sunbul M. Nat Commun. 2023 Jun 30;14(1):3879. doi: 10.1038/s41467-023-39611-1. 10.1038/s41467-023-39611-1 PubMed 37391423