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Addgene

pAV-U6+27-Tornado-biRhoBAST
(Plasmid #196344)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196344 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tornado-biRhoBAST
  • Species
    Synthetic
  • Promoter U6+27

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cloned using pAV-U6+27-Tornado-Broccoli(#124360) . Please visit https://www.biorxiv.org/content/10.1101/2021.11.02.466936v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAV-U6+27-Tornado-biRhoBAST was a gift from Murat Sunbul (Addgene plasmid # 196344 ; http://n2t.net/addgene:196344 ; RRID:Addgene_196344)

  • For your References section:

    Avidity-based bright and photostable light-up aptamers for single-molecule mRNA imaging. Buhler B, Schokolowski J, Benderoth A, Englert D, Grun F, Jaschke A, Sunbul M. Nat Chem Biol. 2023 Apr;19(4):478-487. doi: 10.1038/s41589-022-01228-8. Epub 2023 Jan 19. 10.1038/s41589-022-01228-8 PubMed 36658339
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