pAV-U6+27-Tornado-RhoBAST
(Plasmid
#196341)
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PurposeOverexpression of circular RhoBAST
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196341 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTornado-RhoBAST
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SpeciesSynthetic
- Promoter U6+27
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site SacII (unknown if destroyed)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned using pAV-U6+27-Tornado-Broccoli(#124360)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV-U6+27-Tornado-RhoBAST was a gift from Murat Sunbul (Addgene plasmid # 196341 ; http://n2t.net/addgene:196341 ; RRID:Addgene_196341) -
For your References section:
Super-resolution RNA imaging using a rhodamine-binding aptamer with fast exchange kinetics. Sunbul M, Lackner J, Martin A, Englert D, Hacene B, Grun F, Nienhaus K, Nienhaus GU, Jaschke A. Nat Biotechnol. 2021 Jun;39(6):686-690. doi: 10.1038/s41587-020-00794-3. Epub 2021 Feb 11. 10.1038/s41587-020-00794-3 PubMed 33574610