pGA-red-mini
(Plasmid
#196338)
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Purpose(Empty Backbone) Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 196338 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGA-red-mini
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Backbone manufacturerIndBioTech Lab
- Backbone size (bp) 3534
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Modifications to backboneThe backbone was synthetically assembled from parts of other plasmids. It bears a mRFP1 fluorescent protein placeholder that is excided upon cloning
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Vector typeSynthetic Biology ; Golden Gate Assembly backbone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid was obtained removing URA3 region from pGA-red-maxi.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGA-red-mini was a gift from Paola Branduardi (Addgene plasmid # 196338 ; http://n2t.net/addgene:196338 ; RRID:Addgene_196338) -
For your References section:
Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae. Maestroni L, Butti P, Milanesi R, Pagliari S, Campone L, Serra I, Branduardi P. ACS Synth Biol. 2023 Apr 14. doi: 10.1021/acssynbio.3c00015. 10.1021/acssynbio.3c00015 PubMed 37058502