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Addgene

pGA-red-maxi
(Plasmid #196337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGA-red-maxi
  • Backbone manufacturer
    IndBioTech Lab
  • Backbone size (bp) 6605
  • Modifications to backbone
    The backbone was synthetically assembled from parts of other plasmids. It bears a mRFP1 fluorescent protein placeholder that is excided upon cloning
  • Vector type
    Synthetic Biology ; Golden Gate Assembly backbone
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid was synthetically assembled from parts of other vectors: mRFP1 fluorescent protein plasmid (a gift from Macarena Larroude (Addgene plasmid #120731)); YCplac33 (Gietz and Akio, 1988).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGA-red-maxi was a gift from Paola Branduardi (Addgene plasmid # 196337 ; http://n2t.net/addgene:196337 ; RRID:Addgene_196337)

  • For your References section:

    Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae. Maestroni L, Butti P, Milanesi R, Pagliari S, Campone L, Serra I, Branduardi P. ACS Synth Biol. 2023 Apr 14. doi: 10.1021/acssynbio.3c00015. 10.1021/acssynbio.3c00015 PubMed 37058502
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